1. National Institute of Metrology, Beijing 100013, China
2. Science and Technology Development Center, The Ministry of Agriculture, Beijing 100122, China
3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
4. College of Life Science, China Jiliang University, Hangzhou, Zhejiang 310018, China
Abstract:For 3 kinds of maize matrix reference materials with different genetically modified MON810 maize contents, a multi-laboratory verification for their Q-PCR values is organized. The uncertainties of the multi-laboratory values are evaluated. By weighted average, the Q-PCR values and uncertainties of A, B and C levels of the genetically modified MON810 maize matrix reference materials are 0.51%±0.10%, 1.1%±0.2%, 4.7%±0.7% (k=2), respectively.
[1]中国农作物病虫害编委会.中国农作物病虫害(上册)[M].北京:农业出版社,1979.
[2]Mazzara M, Grazioli E, Savini C, et al. Report on the Verification of the Performance of a MON 810 Event-specific Method on Maize Line MON 810 Using Real-time PCR [R].http://gmo-crl.jrc.ec.europa.eu/gmomethods.htm,2011-06-15.
[3]JJG 1006-94,一级标准物质[S].
[4]European Union Network for GMO Laboratories (ENGL).Definition of minimum performance requirements for analytical methods of GMO testing [R].http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm,2011-06-10.
[5]全浩,韩永志.标准物质及其应用技术[M].第二版.北京:中国标准出版社,2003.