利用微流控技术和量子点技术,将量子点与PDMS均匀混合并压入毛细管中固化成型,研制成量子点掺杂荧光凝胶标准试纸条。通过对量子点浓度的控制,制备了高、中、低共3种荧光强度的荧光凝胶标准试纸条,并对试纸条的光漂白性、均匀性、稳定性等方面进行了考察,评估了不确定度。结果表明该荧光凝胶标准试纸条具有良好的抗光漂白性、均匀性,可在室温避光下稳定保存180天。通过对多家荧光免疫分析仪进行校准实验,证明了该荧光凝胶试纸条具有良好的适用性,为荧光免疫分析仪的校准提供了可靠的参考依据。
Abstract
Quantum dot-doped fluorescent gel reference strips were developed based on microfluidics and quantum dot technology. These strips were formed by uniformly mixed quantum dots and PDMS, which were pressed into capillary tubes to solidify. By controlling the concentration of quantum dots, fluorescent gel reference strips with three fluorescence intensities of high, medium, and low were prepared. The photobleaching, homogeneity, and stability of the reference strips were investigated, and the uncertainties were evaluated. The results showed that the fluorescent gel reference strips possessed good photobleaching resistance and uniformity, and can be stored stably for 180 days at room temperature in the dark. Calibration experiments on a number of fluorescent immunoassay analyzers proved that the fluorescent gel reference strips had good applicability and provided a reliable reference for the calibration of fluorescent immunoassay analyzers.
关键词
计量学 /
荧光凝胶标准试纸条 /
荧光免疫分析仪 /
量子点掺杂 /
微流控技术
Key words
metrology /
fluorescent gel reference strip /
fluorescence immunoassay analyzer;quantum dot-doped;microfluidics
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基金
上海市计量测试技术研究院青年科技创新项目(H00RY2305)
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