量子点掺杂荧光凝胶标准试纸条研制及荧光免疫分析仪校准应用

doi:10.3969/j.issn.1000-1158.2023.12.19

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摘要利用微流控技术和量子点技术,将量子点与PDMS均匀混合并压入毛细管中固化成型,研制成量子点掺杂荧光凝胶标准试纸条。通过对量子点浓度的控制,制备了高、中、低共3种荧光强度的荧光凝胶标准试纸条,并对试纸条的光漂白性、均匀性、稳定性等方面进行了考察,评估了不确定度。结果表明该荧光凝胶标准试纸条具有良好的抗光漂白性、均匀性,可在室温避光下稳定保存180天。通过对多家荧光免疫分析仪进行校准实验,证明了该荧光凝胶试纸条具有良好的适用性,为荧光免疫分析仪的校准提供了可靠的参考依据。

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	陶晴
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关键词 ：计量学, 荧光凝胶标准试纸条, 荧光免疫分析仪, 量子点掺杂, 微流控技术

Abstract：Quantum dot-doped fluorescent gel reference strips were developed based on microfluidics and quantum dot technology. These strips were formed by uniformly mixed quantum dots and PDMS, which were pressed into capillary tubes to solidify. By controlling the concentration of quantum dots, fluorescent gel reference strips with three fluorescence intensities of high, medium, and low were prepared. The photobleaching, homogeneity, and stability of the reference strips were investigated, and the uncertainties were evaluated. The results showed that the fluorescent gel reference strips possessed good photobleaching resistance and uniformity, and can be stored stably for 180 days at room temperature in the dark. Calibration experiments on a number of fluorescent immunoassay analyzers proved that the fluorescent gel reference strips had good applicability and provided a reliable reference for the calibration of fluorescent immunoassay analyzers.

Key words： metrology fluorescent gel reference strip fluorescence immunoassay analyzer;quantum dot-doped;microfluidics

收稿日期: 2023-05-08 发布日期: 2023-12-27

PACS:

TB99

基金资助:上海市计量测试技术研究院青年科技创新项目(H00RY2305)

通讯作者: 闻艳丽(1984-),湖北宜昌人,上海市计量测试技术研究院正高级工程师,主要从事生物计量方面的研究工作。Email:wenyl@simt.com.cn E-mail: wenyl@simt.com.cn

作者简介: 陶晴(1996-),上海人,上海市计量测试技术研究院助理工程师,主要从事生物计量方面的研究工作。Email: taoq@simt.com.cn

引用本文:

陶晴,王乐乐,彭涛,李兰英,刘刚,闻艳丽. 量子点掺杂荧光凝胶标准试纸条研制及荧光免疫分析仪校准应用[J]. 计量学报, 2023, 44(12): 1919-1924.
TAO Qing,WANG Le-le,PENG Tao,LI Lan-ying,LIU Gang,WEN Yan-li. Development of Quantum Dot-Doped Fluorescent Gel Reference Strip and Its Application in Calibration of Fluorescence Immunoassay Analyzer. Acta Metrologica Sinica, 2023, 44(12): 1919-1924.

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