Abstract：Surface plasmon resonance (SPR) is a physical phenomenon observed when sparse and dense electron oscillations occur on a metal surface upon interaction with an incident electromagnetic wave.The analysis of the resonance angle during SPR provides valuable insights into intermolecular interactions.SPR-based calibration free concentration analysis (CFCA) enables the absolute measurement of protein activity concentration by quantifying the diffusion binding rate between a protein and its receptor or antibody under controlled mass-transfer-restricted conditions, eliminating the requirement for reference material, which is a technique for the absolute measurement of protein activity concentration. By reviewing the principle of SPR technology for the absolute quantification of protein activity concentration and its applications in various fields, the advantages and current issues of this technique are discussed, the future development direction of this technology and its main applications in protein quantification are also prospected.
罗世闻,武利庆,杨彬,刘亚辉. 表面等离子体共振技术及其在蛋白质活性浓度绝对定量中的应用[J]. 计量学报, 2023, 44(11): 1770-1775.
LUO Shi-wen,WU Li-qing,YANG Bin,LIU Ya-hui. Surface Plasmon Resonance and its Application in Absolute Quantification of Active Protein Concentration. Acta Metrologica Sinica, 2023, 44(11): 1770-1775.
Pol E, Roos H, Markey F, et al. Evaluation of calibration-free concentration analysis provided by Biacore? systems［J］. Analytical Biochemistry, 2016, 510: 88-97.
［16］
DOrazio P. Biosensors in clinical chemistry—2011 update［J］. Clinica Chimica Acta, 2011,412 (19-20): 1749-1761.
［2］
Chirgadze Y N, Boshkova E A, Kargatov A M, et al. Functional identification of ‘hypothetical protein’ structures with unknown function ［J］. Journal of Biomolecular Structure and Dynamics, 2022, 11 (6): 1-5.
［3］
Chen H, Cai Y, Ji C, et al. AdaPPI: identification of novel protein functional modules via adaptive graph convolution networks in a protein-protein interaction network［J］. Briefings in Bioinformatics, 2023, 24 (1): 118-129.
［6］
Luo Y, Wu L Q, Yang B, et al. A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism ［J］. Scientific Reports, 2018, 8 (1): 7390-7401.
［9］
Pol E. The importance of correct protein concentration for kinetics and affinity determination in structure-function analysis［J］. Journal of Visualized Experiments, 2010,37: 1746-1753.
［11］
Zhang Z, Chu F H, Guo Z, et al. Design and Optimization of Surface Plasmon Resonance Sensor Based on Polymer-Tipped Optical Fiber ［J］. Journal of Lightwave Technology, 2019, 37 (11): 2820-2827.
［12］
Schawaller M, Rhyner C, Wiki M, et al. Evanescence wave-based technology for the rapid and sensitive quantification of biological analytes［J］. Journal of Allergy and Clinical Immunology, 2018,141 (2): 817- 820.
［14］
Huang Y, Wang Y, Xu G, et al.Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber ［J］. Biosensors, 2022,12 (3): 141-156.
［19］
Chiu N F. The Current Status and Future Promise of SPR Biosensors［J］. Biosensors (Basel), 2022,12 (11): 456-468.
［34］
Visentin J, Minder L, Lee J, et al. Calibration free concentration analysis by surface plasmon resonance in a capture mode ［J］. Talanta, 2016, 148: 478-485.
Yang H M. Biometrics development message ［J］. Acta Metrologica Sinica, 2023, 44 (3): 314.
［1］
JJF 1265—2010, 生物计量术语及定义 ［S］.
［10］
Prabowo B A, Purwidyantri A, Liu K C. Surface Plasmon Resonance Optical Sensor: A Review on Light Source Technology ［J］. Biosensors (Basel), 2018,8 (3): 80-89.
［20］
Moraskie M, Roshid M, OConnor G, et al. Microbial whole-cell biosensors: Current applications, challenges, and future perspectives ［J］. Biosens Bioelectron, 2021, 191 (1): 3359-3368.
［22］
Liu Y, Zhang X. Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays ［J］. Micromachines (Basel), 2021, 12 (7): 657-669.
［24］
Su P, He Z, Wu L, et al. SI-traceable calibration-free analysis for the active concentration of G2-EPSPS protein using surface plasmon resonance［J］. Talanta, 2018, 178 (1): 78-84.
［26］
Sissoff L, Mousli M, England P, et al. Stable trimerization of recombinant rabies virus glycoprotein ectodomain is required for interaction with the p75NTR receptor ［J］. Journal of General Virology, 2005, 86 (9): 2543-2552.
［29］
Ray S, Kumar V, Bhave A, et al. Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity ［J］. Journal of Proteomics, 2015, 127: 103-113.
［30］
Aniol-Nielsen C, Toft-Hansen H, Dahlback M, et al. Calibration-free concentration analysis for quantification of anti-drug specific antibodies in polyclonal positive control antibodies and in clinical samples ［J］. Journal of Immunological Methods, 2021,497: 113002.
［32］
Behr S, Heermann R, Jung K. Insights into the DNA-binding mechanism of a LytTR-type transcription regulator ［J］. Bioscience Reports, 2016,36 (2): e00326.
［7］
Duffy D C. Digital detection of proteins［J］. Lab on a Chip, 2023, 23 (5): 818-847.
［17］
Justino C, Duarte A C, Rocha-Santos T. Critical overview on the application of sensors and biosensors for clinical analysis［J］. Trends Analyt Chem, 2016,85 (1): 36-60.
Li J L.Wu L Q, Jin Y X, et al. Determination of the Mass Fraction of Pure Human Serum Albumin by HPLC-IDMS ［J］. Acta Metrologica Sinica, 2016,37 (3): 328-332.
［15］
Dhara P, Kumar R, Binetti L, et al. Optical Fiber-Based Heavy Metal Detection Using the Localized Surface Plasmon Resonance Technique ［J］. IEEE sensors journal, 2019,19 (19): 8720-8726.
［25］
Hu T, Wu L, Sun X, et al. Comparative study on quantitation of human myoglobin by both isotope dilution mass spectrometry and surface plasmon resonance based on calibration-free analysis ［J］. Anal Bioanal Chem, 2020, 412 (12): 2777-2784.
［35］
杨焕明. 生物计量发展寄语 ［J］. 计量学报, 2023, 44 (3): 314.
［13］
Zhou Y, Zhang Y, Han B, et al. Biochemical sensor based on functional material assisted optical fiber surface plasmon resonance: A review ［J］. Measurement, 2023,207 (1): 112353-112368.
［23］
Lertvachirapaiboon C, Baba A, Ekgasit S, et al. Transmission surface plasmon resonance techniques and their potential biosensor applications［J］. Biosens Bioelectron, 2018, 99 (1): 399-415.
［33］
Abdiche Y N, Yeung Y A, Chaparro-Riggers J, et al. The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity ［J］. mAbs, 2015, 7 (2): 331-343.
［18］
Baracu A M, Dinu Gugoasa L A. Review—Recent Advances in Microfabrication, Design and Applications of Amperometric Sensors and Biosensors ［J］. Journal of the Electrochemical Society, 2021,168 (3): 37503-37519.
［21］
Nguyen H, Park J, Kang S, et al.Surface Plasmon Resonance: A Versatile Technique for Biosensor Applications ［J］. Sensors, 2015, 15 (5): 10481-10510.
［28］
Grover S V, Ray S, Karlsson R, et al. Calibration-free concentration analysis of protein biomarkers in human serum using surface plasmon resonance ［J］. Talanta, 2015,144 (1): 801-808.
［31］
Karlsson R. Biosensor binding data and its applicability to the determination of active concentration ［J］. Biophysical reviews, 2016,8 (4): 347-358.
2023, Vol. 44 
