数字PCR法在食品检测标准物质研制中的应用

范宏博; 蔡永洪; 李月玥; 黄满英; 张力玲; 马婷婷; 胡松青; 胡良勇

doi:10.3969/j.issn.1000-1158.2023.03.21

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计量学报 ›› 2023, Vol. 44 ›› Issue (3) : 472-478. DOI: 10.3969/j.issn.1000-1158.2023.03.21

电离辐射、标准物质与生物计量

数字PCR法在食品检测标准物质研制中的应用

范宏博^1,2,蔡永洪¹,李月玥¹,黄满英¹,张力玲¹,马婷婷¹,胡松青²,胡良勇¹

作者信息 +

Application of Digital PCR in Development of Food Detection Reference Materials

FAN Hong-bo^1,2,CAI Yong-hong¹,LI Yue-yue¹,HUANG Man-ying¹,ZHANG Li-ling¹,MA Ting-ting¹,HU Song-qing²,HU Liang-yong¹

Author information +

文章历史 +

摘要

食品检测标准物质是保证食品检测量值溯源性、互认性和准确性的测量标准,其研制需要精准的定值方法。数字PCR法是灵敏、准确和抗基质干扰强的核酸绝对定量方法,已在医疗诊断、转基因检测和动物疫病检测等众多领域广泛应用。对数字PCR法进行介绍,综述其在转基因食品检测、动植物源性分析和食源性致病菌检测标准物质研制中的研究进展,以期为食品检测核酸标准物质的研制提供参考。

Abstract

Food detection reference material is a measuring instrument to ensure traceability, mutual recognition and accuracy of food detection value, and its development needs accurate determination methods. Digital PCR is a sensitive, accurate and matrix-resistant method for absolute quantification of nucleic acid, which is widely applied in many fields such as medical diagnosis, genetically modified organism detection and animal disease detection. Digital PCR method and its research progress in the detection of genetically modified foods, animal and plant origin analysis and the development of food-borne pathogenic bacteria detection reference materials is reviewed to provide reference for the development of food detection nucleic acid reference materials.

导出引用

范宏博,蔡永洪,李月玥,黄满英,张力玲,马婷婷,胡松青,胡良勇. 数字PCR法在食品检测标准物质研制中的应用[J]. 计量学报. 2023, 44(3): 472-478 https://doi.org/10.3969/j.issn.1000-1158.2023.03.21

FAN Hong-bo,CAI Yong-hong,LI Yue-yue,HUANG Man-ying,ZHANG Li-ling,MA Ting-ting,HU Song-qing,HU Liang-yong. Application of Digital PCR in Development of Food Detection Reference Materials[J]. Acta Metrologica Sinica. 2023, 44(3): 472-478 https://doi.org/10.3969/j.issn.1000-1158.2023.03.21

中图分类号： TB99

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