Abstract：DNA methylation is the most important epigenetic mechanism that has been deeply studied. DNA methylation analysis based on sequencing has laid the foundation for describing a relatively complete DNA CpG map. In the past few decades, the emergence of a large number of DNA methylation measurement technologies has greatly improved the research of genome methylation analysis. However, due to the great differences in equipment and personnel operations of different laboratories, it is difficult to compare the measurement results between laboratories. At the same time, due to the lack of standard analysis procedures and reference materials for evaluating genome methylation, it is not clear whether the current methylation specificity is as accurate as the usual recognition sequence implies. Therefore, the development and problems of DNA methylation measurement technology are discussed, and the importance of DNA methylation measurement accuracy evaluation and reference materials is proposed.
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2022, Vol. 43 
