2019新型冠状病毒的抗原抗体检测

高原; 陈川; 王晶

doi:10.3969/j.issn.1000-1158.2020.05.01

PDF(464 KB)

计量学报 ›› 2020, Vol. 41 ›› Issue (5) : 513-517. DOI: 10.3969/j.issn.1000-1158.2020.05.01

电离辐射、标准物质与生物计量

2019新型冠状病毒的抗原抗体检测

高原,陈川,王晶

作者信息 +

Antigen and Antibody Detection of SARS-CoV-2

GAO Yuan,CHEN Chuan,WANG Jing

Author information +

文章历史 +

摘要

2019新型冠状病毒(SARS-CoV-2)入侵人体引起急性呼吸道传染病,命名为2019冠状病毒病(COVID-19)。新冠病毒在全球范围传播对全球公共卫生安全构成了巨大的威胁,新冠病毒的检测对疫病诊断尤为重要。免疫分析技术作为核酸检测的辅助手段,主要用于对疑似病例和与感染者密切接触人员的筛查。免疫诊断技术包括抗体和抗原检测,通过检测感染部位或者血液样本中是否含有病毒本身的蛋白或是由病毒引起免疫反应而产生的抗体来判断是否携带病毒。对目前用于新冠病毒抗原和抗体检测的免疫分析方法着重进行了分析。

Abstract

2019 novel coronavirus (SARS-CoV-2) invades the human body and causes an acute respiratory infectious disease, which was named as Corona Virus Disease (COVID-19). The global spread of SARS-Cov-2 poses a huge threat to global public health security. The detection of SARS-CoV-2 is particularly important for disease diagnosis. Immunoassay method has played a role in clinical diagnosis as an aid to virus nucleic acid detection. Immuno-diagnostic techniques include antibody and antigen detection, and determine whether the virus are carried by detecting the viruss own protein and antibody. Analysis was focused on the current immunoassay methods for SARS-CoV-2.

导出引用

高原,陈川,王晶. 2019新型冠状病毒的抗原抗体检测[J]. 计量学报. 2020, 41(5): 513-517 https://doi.org/10.3969/j.issn.1000-1158.2020.05.01

GAO Yuan,CHEN Chuan,WANG Jing. Antigen and Antibody Detection of SARS-CoV-2[J]. Acta Metrologica Sinica. 2020, 41(5): 513-517 https://doi.org/10.3969/j.issn.1000-1158.2020.05.01

中图分类号： TB99

参考文献

［1］Ramaiah A, Arumugaswami V. Insights into Cross-species Evolution of Novel HumanCoronavirus 2019-nCoV and Defining Immune Determinants for Vaccine Development［J］. bioRxiv, 2020. https://doi.org/10.1101/2020.01.29.925867.
［2］张永卓, 王晶, 傅博强, 等. 2019新型冠状病毒的核酸检测［J］.计量学报, 2020, 41(4): 393-398.
Zhang Y Z, Wang J,Fu B Q, et al. Nucleic Acid Detection of the SARS-CoV-2［J］.Acta Metrologica Sinica, 2020,41(4): 393-398.

［3］Shen M Z, Zhou Y, Ye J W, et al. Recent advances and perspectives of nucleic acid detection forcoronavirus［J］. Journal of Pharmaceutical Analysis, 2020. https://doi.org/10.1016/j.jpha.2020.02.010.
［4］Peng L, Liu J, Xu W X, et al. 2019 Novel Coronavirus can be detected in urine, blood, anal swabs and oropharyngeal swabs samples［J］. medRxiv. https://doi.org/10.1101/2020.02.21.20026179.
［5］Zhou P, Yang X L, Wang X G, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin ［J］. Nature, 2020, 579: 270-273.
［6］Xiao S Y, Wu Y, Liu H. Evolving status of the 2019 novel coronavirus infection: Proposal of conventional serologic assays for disease diagnosis and infection monitoring ［J］. Journal of Medical Virology, 2020, 92(5):1-4.
［7］Jia X, Zhang P, Tian Y. Clinical significance of IgM and IgG test for diagnosis of highly suspected COVID-19 infection［J］. medRxiv. https://doi.org/10.1101/2020.02.28.20029025.
［8］Zhao J, Yuan Q, Wang H, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019［J］. medRxiv. https://doi.org/10.1101/2020.03.02.20030189.
［9］Zhang J, Liu J, Li N, et al. Serological detection of 2019-nCoV respond to the epidemic: A useful complement to nucleic acid testing［J］. medRxiv. https://doi.org/10.1101/2020.03.04.20030916.
［10］Cai X, Chen J, Hu J, et al. A Peptide-based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Corona Virus Disease 2019 (COVID-19) ［J］. medRxiv.https://doi.org/10.1101/2020.02.22.20026617.
［11］Li M, Jin R, Peng Y, et al. Generation of antibodies against COVID-19 virus for development of diagnostic tools ［J］. medRxiv. https://doi.org/10.1101/2020.02.20.20025999.
［12］Wan Y S, Shang J, Graham R, et al. Receptor recognition by novel coronavirus from Wuhan: An analysis based on decade-long structural studies of SARS ［J］. Journal of Virology,2020,doi:10. 1128/JVI. 00127-20.

［13］Lei C H, Fu W Y, Qian K W, et al. Potent neutralization of 2019 novel coronavirus by recombinant ACE2-Ig ［J］. bioRxiv.https://doi.org/10.1101/2020.02.01.929976.
［14］Tian X L, Li C, Huang A L, et al. Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody［J］. bioRxiv. https://doi.org/10.1101/2020.01.28.923011.

［15］Xiang J, Yan M, Li H, et al. Evaluation of Enzyme-Linked Immunoassay and Colloidal GoldImmunochroma-tographic Assay Kit for Detection of Novel Coronavirus (SARS-Cov-2) Causing an Outbreak of Pneumonia (COVID-19) ［J］. medRxiv. https://doi.org/10.1101/2020.01.29.925867.