1.College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China
2.National Institute of Metrology, Beijing 100029, China
3.Optoelectronics College, Beijing Information Science and Technology University, Beijing 100192, China
Abstract：The accuracy of the data measured by the fluorescence microscope is influenced by the linear dynamic range, the uniformity of the light field, and the stability of the light source. Fluorescence microscopy experiments were carried out on fluorescent microscopy materials, and it was found that fluorescence characteristics such as fluorescence linearity, uniformity and stability are important for fluorescence microscopy analysis. The spectral characteristics, linearity, uniformity, and light stability of fluorescent film materials were studied, a method for measuring the fluorescent properties of fluorescent film materials was established, a preliminary exploration of the calibration method for fluorescent microscopes was made, and the foundation for the calibration of fluorescent microscopes was laid.
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2019, Vol. 40 
