Abstract：Uncertainties from the determination procedure of vitamin D2 and vitamin D3 in "Safety and Technical Standards for Cosmetics"(2015 Edition) by high performance liquid chromatography (HPLC) were assessed. The mathematical model was established, and each source and uncertainty component were analyzed. The combined standard uncertainty and expanded uncertainty were reported at last. The results showed that the expanded uncertainties were 10.36 μg/g and 9.71 μg/g (95% confidence level, k=2) when the content of Vitamin D2 and Vitamin D3 in cosmetics were 48.84 μg/g and 45.76 μg/g. The main sources for the uncertainty were discussed, and suggestions for improvement were also introduced.
王涛,刘鹏鸣,杜晖,范菲,杨会鸽,李静静,郑妍. 化妆品中维生素D2、维生素D3含量的测量不确定度评定[J]. 计量学报, 2019, 40(4): 728-735.
WANG Tao,LIU Peng-ming,DU Hui,FAN Fei,YANG Hui-ge,LI Jing-jing,ZHENG Yan. Evaluation of Measurement Uncertainty in the Determination of Vitamin D2 and Vitamin D3 in Cosmetics. Acta Metrologica Sinica, 2019, 40(4): 728-735.
［1］余学锋,于杰,王柯,等.基于证据理论的测量不确定度评定与分析［J］.计量学报, 2017, 38(2):252-256.
Yu X F, Yu J,Wang K, et al. Evaluating and Analyzing Measurement Uncertainty Based on the Theory of Evidence
［J］. Acta Metrologica Sinica, 2017, 38(2):252-256.
［2］叶德培. 测量不确定度理解评定与应用［M］. 北京:中国质检出版社, 2013.
［3］Mousa A, Naderpoor N, Teede H, et al. Vitamin D Supplementation for Improvement of Chronic Low-grade Inflammation in Patients with Type 2 Diabetes: a Systematic Review and Meta-Analysis of Randomized Controlled Trials.［J］. Nutrition Reviews, 2018,76(5):380-394.
［4］Tripkovic L, Wilson L R, Lanham-New S A. vitamin D2 vs. vitamin D3: They are not one and the same［J］. Nutrition Bulletin, 2017, 42(4):331-337.
［5］Prasad P, Kochhar A. Interplay of vitamin D and metabolic syndrome: A review［J］. Diabetes & Metabolic Syndrome Clinical Research & Reviews, 2016, 10(2):105-112.
［6］Tarn D M, Paterniti D A, Wenger N S. Provider Recommendations in the Face of Scientific Uncertainty: An Analysis of Audio-Recorded Discussions about Vitamin D［J］. Journal of General Internal Medicine, 2016, 31(8):1-9.
［7］Forouhi N G, Menon R K, Sharp S J, et al. Effects of vitamin D2 or D3 supplementation on glycaemic control and cardiometabolic risk among people at risk of type 2 diabetes: results of a randomized double-blind placebo-controlled trial［J］. Diabetes Obesity & Metabolism, 2016, 18(4):392-400.
［8］Adamec J, Jannasch A, Huang J, et al. Development and optimization of an LC-MS/MS-based method for simultaneous quantification of vitamin D2, vitamin D3, 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3［J］. Journal of Separation Science, 2011, 34(1):11-20.
［9］霍艳敏, 王骏, 段文增,等. HPLC-APCI(+)-MS/MS测定乳制品中的维生素D2及D3［J］. 分析测试学报, 2016, 35(3):327-331.
Huo Y M, Wang J, Duan W Z, et al. Determination of Vitamin D2 and Vitamin D3 in Dairy Products by HPLC-APCI (+)-MS/MS［J］. Journal of Instrumental Analysis, 2016, 35(3):327-331.
［10］胡代花, 张嘉昕, 韩豪, 等.UPLC法快速测定婴儿维生素D3滴剂、维生素D3片、维生素AD滴剂及维生素D2片中维生素D3和维生素D2［J］. 药物分析杂志, 2016,36(8):1409-1414.
Hu D H, Zhang J X, Han H, et al. Rapid Determination of Vitamin D3, D2 in Baby Vitamin D3 Drops, Vitamin D3 Tablets, Vitamin AD Drops and Vitamin D2 Tablets by UPLC［J］. Chinese Journal of Pharmaceutical Analysis, 2016, 36(8):1409-1414.
［11］卢金淼, 李智平. 补充维生素D2与维生素D3的应用评价［J］. 世界临床药物, 2013, 34(9): 566-569.
Lu J M, Li Z P. Comparative Evaluation of Vitamin D2 and Vitamin D3［J］. World Clinical Drugs, 2013, 34(9): 566-569.
［12］朱英, 杨艳伟,王歆. 化妆品中维生素D2、维生素D3的高效液相色谱测定［J］. 卫生研究, 2005, 34(5): 624-625.
Zhu Y, Yang Y W, Wang X. Determination of Vitamins D2, Vitamin D3 in Cosmetics by High Performance Liquid Chromatography［J］. Journal of Hygiene Research, 2005, 34(5):624-625.
［13］化妆品安全使用规范(2015年版)［S］. 2016.
［14］JJF 1059.1—2012 测量不确定度评定与表示［S］. 2012.
［15］JJG 196—2006常用玻璃量器［S］. 2006.
［16］JJG 10—2005专用玻璃量器［S］. 2005.
2019, Vol. 40 
