Determination of Biological Toxicity of Medical Institutions Waste Water by the Method of Luminescent Bacteria
LI Yong-liang1,LI Jian1,LI Gui-lian2,YU Ting-ting3
1. Jiamusi Environmental Monitoring Station, Jiamusi, Heilongjiang 154004, China
2. Department of Environmental Science and Engineering, Heilongjiang University, Harbin, Heilongjiang 150080, China
3. Department of Applied Chemistry, Qiqihar University, Qiqihar, Heilongjiang 161006, China
Abstract:Vibrio Fischeri as spawn, a comprehensive test of the waste water discharged by medical institutions was carried out, combine with the determination of fecal coliform. Monitoring results of fecal flora of waste water conformed to the requirements of the corresponding standard limit, but medical waste water with biological toxicity generated certain biological inhibition rate to photogenic bacterium. The waste water of infectious diseases and tuberculosis medical institutions possessed biological inhibition rates as high as 44.7%and 49.8%. The inhibition rate of waste water from traditional Chinese medicine medical institutions caused a minmum inhibition rate of 8.1%. The experimental results showed that the luminescent bacteria method could be applied to the determination of waste water of medical institutions.
李永亮,李健,李桂莲,于亭亭. 发光菌法测定医疗机构废水生物毒性[J]. 计量学报, 2017, 38(2): 238-241.
LI Yong-liang,LI Jian,LI Gui-lian,YU Ting-ting. Determination of Biological Toxicity of Medical Institutions Waste Water by the Method of Luminescent Bacteria. Acta Metrologica Sinica, 2017, 38(2): 238-241.
[1]Jennings V L K,Brandes M H R,Bird D J. Assessing chemical toxicity with the bioluminescent photobacterium(Vibrio fischeri):a comparison of three commercial systems[J]. Water Research, 2001, 35(14):3448-3456.
[2]周世明,舒为群,赵清.发光菌在水环境污染物检测中的应用研究进展[J].环境与健康杂志,2007, 24(10):837-839.
[3]朱文杰,汪杰.发光细菌一新种——青海弧菌[J]. 海洋与湖沼,1994,25(3):273-279.
[4]GB/T 15441—1995 水质 急性毒性的测定发光细菌法[S].
[5] DIN EN ISO 11348-1:2009 Water quality—Determination of the inhibitory effect of water samples on the light emission of vibrio fischeri (luminescent bacteria test). Part 1: Method using freshly prepared bacteria second edition[S]. 2009.
[6]李健,李永亮,李桂莲,等.发光菌法测定松花江佳木斯江段水质生物毒性的研究[J].环保科技,2013,19(3):34-36.
[7]薛蕾,林婧,隋志伟,等.大肠杆菌定量检测用标准物质的研制[J].计量学报, 2015, 36(6):652-656.
[8]国家环境保护总局. 水和废水监测分析方法[M]. 4版增补版. 北京:中国环境科学出版社,2002.
[9]GB 18466—2005 医疗机构水污染物排放标准[S].