Abstract：The quantum efficiency of fluorescence is the ratio of photons emitted and absorbed, and is the key parameter to characterize the properties of fluorescent materials. However, if the optical path and detector used for absolute measurement of fluorescence quantum efficiency are not calibrated and traced back or the calibration method is not correct, it will cause the inaccurate measurement spectrum and further cause the inaccurate calculation results of fluorescence quantum efficiency. The mercury argon lamp is used to calibrate the monochromator to ensure the accuracy of excitation wavelength and emission wavelength. The standard radiation source is used to calibrate the spectral relative intensity of the light path, the monochromators of the emission unit and the detector, which ensures the accuracy of the spectral relative intensity of the excitation band and the emission band. Finally, the uncertainty of measurement is analyzed based on the measurement model, the results show that the relative standard uncertainty is 3.58%, the relative expanded uncertainty is 7.16%,k=2 in the excitation band of 300~360nm and the emission band of 370~900nm. By calibrating the monochromators and the spectral relative intensity, it can provide a reference for the accurate measurement of fluorescence quantum efficiency.
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2022, Vol. 43 
