Hebei Province Key Laboratory of Measurement Technology and Instrument , Institute of Electric Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China
Abstract:Three kinds of quinolones (fluoroquinoline, enrofloxacin and levofloxacin) in honey were analyzed by three-dimensional fluorescence spectroscopy. The spectra were pretreated to eliminate the second-order Rayleigh scattering and Raman scattering, and the spectral noise was eliminated by wavelet optimization set ensemble empirical mode decomposition (EEMD). The bilinear least square/residual bilinear (BLLS/RBL) algorithm was used to detect the samples before and after pretreatment. The results show that after pretreatment, the spectrum of each component in the sample can be accurately resolved, and it has a high similarity with the original spectrum. In the quantitative analysis, the predicted average recovery (RA) of fluoroquinoline, enrofloxacin and levofloxacin is 94.99%, 100.20%, 103.20% respectively, the root mean square error (RMSE) is 4.03, 0.21, 0.29μg/L respectively, the sensitivity (SL) is 3.2×103, 3.5×104, 3.3×104μg/L, respectively; and the detection lower limit (Lout) is 2.08, 0.18, 0.19μg/L respectively, which are superior to the results without pretreatment.
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