以海拉细胞作为研究对象,对细胞数量差异引起的细胞生长速率变化以及对药物效果的影响展开研究。实验结果表明细胞起始数量越多,贴壁后密度越大,生长速率越快,并且药物对其生长的抑制作用越低。因此,准确地细胞计数是生物学实验的关键。该研究进一步比较了3种常用细胞计数方法:细胞计数板法、细胞计数仪法、流式细胞仪法,结果显示其线性相关系数R2分别为0.9835,0.9997和0.9960,同等条件下,细胞计数仪具有最高的准确性。此外,该研究同时也对结晶紫染色、底面积拍照、蛋白质含量估算和蛋白质内参GAPDH测量等间接方法进行评估,为生物学实验过程中的细胞数量测算提供数据参考。
Abstract
Hela cells were used to investigate the changes in cell growth rate and the influence of drug effect induced by the cell number differences at the cell seeding step. The experimental results indicated that the larger the initial number of cells, the higher the density after adhesion, the faster the growth rate, and the lower the inhibitory effect of drugs on its growth. Therefore, the accuracy and efficiency of cell counting are of great importance for in vitro studies. Three commonly used cell counting methods were compared including the cell counting plate method, the cell counting apparatus method, and the flow cytometry method. Their linear regression R2 were 0.9835, 0.9997, and 0.9960 respectively. Under the same condition, the cell counting apparatus method held the highest accuracy. In addition, indirect methods such as crystal violet staining, basal area photography, protein content estimation, and protein reference GAPDH measurement were also evaluated in to provide data reference for cell number measuring in biological experiments.
关键词
计量学;细胞计量;细胞数量;生长速率;细胞计数板法 /
细胞计数仪法 /
流式细胞仪法
Key words
metrology /
cell metrology /
cell number /
growth rate /
cell counting plate method /
cell counting apparatus method /
flow cytometry method
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基金
中国计量科学研究院基本科研业务费(AKYZZ2224)
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