生物气溶胶监测仪的校准方法比较

doi:10.3969/j.issn.1000-1158.2022.01.22

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摘要生物气溶胶监测仪利用荧光光谱法检测空气中的生物性物质,对疾病防控以及生物防恐预警意义重大。研究发现目前的国产生物气溶胶监测仪在采集空气中的生物颗粒时并不能区分不同细菌状态,得到的为总细菌数。通过实时定量PCR法和菌落计数法检测BioSampler采集瓶中的微生物,发现总细菌数和可培养的细菌数差异显著。利用扫描电子显微镜和荧光显微镜观察发现,采样器的撞击和离心作用影响了所收集的空气中微生物的生存能力,因此不宜用菌落计数法校准生物气溶胶监测仪。

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	田莹
	张国城
	吴丹
	刘佳琪
	李晶晶
	霍胜伟
	沈上圯
	潘一廷

关键词 ：计量学, 生物气溶胶监测仪, 菌落计数, 实时定量PCR, 荧光显微镜, 扫描电子显微镜

Abstract：The bioaerosol monitor detect biological substances in the air based on laser-induced fluorescence, which is of great significance for disease prevention and early warning of biological terrorism. The study found that the current domestic bioaerosol monitor cant distinguish different bacterial states when collecting biological particles in the air. The bioaerosol monitor detect the total number of bacteria. Through real-time quantitative PCR and colony counting method to detect the microorganisms in the BioSampler, it was found that the total number of bacteria and the number of cultivable bacteria were significantly different. Observation by scanning electron microscope and fluorescence microscope found that the impact and centrifugation of the sampler affected the viability of the microorganisms, so it is not suitable to calibrate the bioaerosol monitor by the colony culture method.

Key words： metrology bioaerosol detector colony counting method real-time quantitative PCR fluorescence microscope scanning electron microscope

收稿日期: 2021-03-11 发布日期: 2022-01-06

PACS:

TB99

基金资助:国家市场监管总局科技计划项目(2021MK003)

通讯作者: 张国城(1980-),男,福建永春人,北京市计量检测科学研究院教授级高工,主要从事化学分析与环境监测仪器的计量检测研究。Email: zhanggc@bjjl.cn E-mail: huosw@bjjl.cn

作者简介: 田莹(1990-),女,河南焦作人,北京市计量检测科学研究院工程师,研究方向为颗粒物相关环境监测仪器。Email: tiany@bjjl.cn

引用本文:

田莹, 张国城, 吴丹, 刘佳琪, 李晶晶, 霍胜伟, 沈上圯, 潘一廷. 生物气溶胶监测仪的校准方法比较[J]. 计量学报, 2022, 43(1): 140-144.
TIAN Ying, ZHANG Guo-cheng, WU Dan, LIU Jia-qi, LI Jing-jing, HUO Sheng-wei, SHEN Shang-yi, PAN Yi-ting. Comparison of Biological Calibration Methods of Bioaerosol Monitor. Acta Metrologica Sinica, 2022, 43(1): 140-144.

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http://jlxb.china-csm.org:81/Jwk_jlxb/CN/10.3969/j.issn.1000-1158.2022.01.22 或 http://jlxb.china-csm.org:81/Jwk_jlxb/CN/Y2022/V43/I1/140

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