基于差动共焦显微技术的微区拉曼光学系统构建与实验研究

姜静子; 高思田; 黄鹭; 李琪; 连笑怡

doi:10.3969/j.issn.1000-1158.2020.04.002

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计量学报 ›› 2020, Vol. 41 ›› Issue (4) : 399-405. DOI: 10.3969/j.issn.1000-1158.2020.04.002

几何量计量

基于差动共焦显微技术的微区拉曼光学系统构建与实验研究

姜静子¹, 高思田², 黄鹭², 李琪², 连笑怡²

作者信息 +

Construction and Experimental Study of Micro-area Raman OpticalSystem Based on Differential Confocal Microscopy Technology

JIANG Jing-zi¹, GAO Si-tian², HUANG Lu², LI Qi², LIAN Xiao-yi²

Author information +

文章历史 +

摘要

研制了一种基于差动共焦显微技术的微区拉曼光学系统装置,对无机样品进行微区拉曼光谱探测。传统显微共焦拉曼光谱技术没有强调系统的定焦能力,而所研制的光学系统装置利用差动共焦曲线过零点与焦点位置精确对应的特性,采用反馈控制技术,具有长时间定焦功能。在微区拉曼散射信号收集时,采用多模光纤空间耦合技术,以光纤代替传统物理探测针孔,提高了环境抗干扰能力,优化了系统结构和装调性能。实验结果表明:该装置具有较高稳定性,可有效探测单壁碳纳米管在1581.510cm^-1、2708.065cm^-1特征峰处的拉曼频移及纯物质硫在153.113cm^-1、219.917cm^-1、473.322cm^-1特征峰处的拉曼频移并且实现碳管的单线检测,满足了光谱探测系统装置设计需求。

Abstract

A micro-area Raman optical system based on differential confocal microscopy technology is developed to detect the micro-area Raman spectroscopy of inorganic samples. The traditional confocal Raman spectroscopy technology does not emphasize the focusing ability of the system, but the optical system device has the function of long-time focusing by using on the characteristic that the zero-crossing point of the differential confocal curve corresponds precisely to the focal point position and adopting feedback control technology. When collecting Raman scattering signals in micro-area, multi-mode optical fiber spatial coupling technology is used to replace traditional physical detection pinholes with optical fiber, which improves the anti-interference ability of the environment and optimizes the system structure and adjusting performance. The experimental results show that the device has high stability and can effectively detect the Raman frequency shift of single-walled carbon nanotubes at 1581.510cm^-1, 2708.065cm^-1 characteristic peaks and the Raman frequency shift of pure sulfur at 153.113cm^-1, 219.917cm^-1, 473.322cm^-1 characteristic peaksand realize the single-line detection of the carbontube. The device meets the design requirements of the spectral detection system.

导出引用

姜静子, 高思田, 黄鹭, 李琪, 连笑怡. 基于差动共焦显微技术的微区拉曼光学系统构建与实验研究[J]. 计量学报. 2020, 41(4): 399-405 https://doi.org/10.3969/j.issn.1000-1158.2020.04.002

JIANG Jing-zi, GAO Si-tian, HUANG Lu, LI Qi, LIAN Xiao-yi. Construction and Experimental Study of Micro-area Raman OpticalSystem Based on Differential Confocal Microscopy Technology[J]. Acta Metrologica Sinica. 2020, 41(4): 399-405 https://doi.org/10.3969/j.issn.1000-1158.2020.04.002

中图分类号： TB92

参考文献

1 RamanC V, KrishnanK S. A new type of secondary radiation［J］. Nature, 1928, 121: 501-502.
2 王雪花, 程明霄, 朱倩, 等. 激光显微拉曼光谱仪的设计与标定［J］. 仪表技术与传感器, 2014, (7): 61-63. WangX H, ChengM X, ZhuQ, et al. Design and Calibration of Laser Micro-Raman Spectrometer［J］. Instrument Technique and Sensor, 2014, (7): 61-63.
3 姚雅萱, 任玲玲, 高思田,等. 拉曼光谱仪相对强度标准物质量值表达及不确定度评定［J］. 计量学报, 2017, 38(3): 376-379. YaoY X, RenL L, GaoS L,et al. Value Expression and Uncertainty Evaluation of Relative Intensity Calibration Reference Material for Raman Spectroscopy［J］. ActaMetrologica Sinica, 2017, 38(3): 376-379.
4 杨永梅. 拉曼光谱技术应用的综述［J］. 科技传播, 2010,5(20):115. YangY M. The Application of Roman Spectroscopy Technology［J］. Public Communication Of Science & Technology, 2010, 5(20): 115.
5 孙素琴, 周群, 张宣, 等. 傅里叶变换拉曼光谱法无损鉴别植物生药材［J］. 分析化学, 2002, 30(2): 140-143. SunS Q, ZhouQ, ZhangX, et al. Undamaged Determination of Raw Plant Medicinal Drugs with Fourier Transform Raman Spectroscopy［J］. Chinese Journal Of Analytical Chemistry, 2002, 30(2): 140-143.
6 倪丹丹, 王伟伟, 姚建林, 等. 可循环表面增强拉曼光谱基底的制备及其应用［J］. 光谱学与光谱分析, 2011, 31(2): 394-397. NiD D, WangW W, YaoJ L,et al. Fabrication of Reproducible Surface Enhanced Raman Scattering Substrate and Its Application［J］. Spectroscopy And Spectral Analysis, 2011, 31(2): 394-397.
7 赵晓杰, 江山, 陆冬生, 等. 激发光源紫外-可见连续可调谐共振拉曼光谱测量系统［J］. 光谱学与光谱分析, 1997, (1): 66-70. ZhaoX J, JiangS, LuD S,et al. A Resonance Raman Spectrometer With UV Visble Continuously Tunable Excitation Lines［J］. Spectroscopy Spectral Analysis, 1997, (1): 66-70.
8 赵维谦, 邱丽荣, 郭俊杰, 等. 差动共焦拉曼光谱测试方法. 中国, ZL200810115601.1［P］. 2008.
9 赵维谦, 崔晗, 邱丽荣, 等. 激光差动共焦图谱显微成像方法与装置:ZL 201310026956.4［P］.2013-05-08.
10 崔晗. 高空间分辨激光差动共焦拉曼光谱成像方法与技术研究［D］. 北京: 北京理工大学, 2016.
11 周昭, 陈和, 张寅超, 等. 微型拉曼光谱仪杂散光分析及抑制方法的改进［J］. 光谱学与光谱分析, 2017, 37(3): 772-777. ZhouZ, ChenH, ZhangY C, et al. The Improved Suppression and Analysis of Stray Light in the Miniature Raman Spectrometer［J］. Spectroscopy and Spectral Analysis, 2017, 37(3): 772-777.
12 JabbourJ M, SalduaM A, BixlerJ N, et al. Confocal endomicroscopy: Instrumentationand medical applications ［J］. Annals of Biomedical Engineering, 2012, 40(2): 378-397.
13 HarrisJ, NorrisG, ConnellG M. Characterization of Periodically Poled Materials Using Nonlinear Microscopy ［J］. Optics Express, 2008, 16(8): 5667~5672.
14 TataB V R, RajB. Confocal Laser Scanning Microscopy: Applications in Material Science and Technology ［J］. Bulletin of Materials Science, 1998, 21(4): 263~278.
15 毛新越, 赵维谦, 王允, 等. 激光差动共焦显微成像中的轴向快速定焦方法［J］. 光学技术, 2015, 41(5): 385-389. MaoX Y, ZhaoW Q, WangY, et al. The method of axial fast identified focus of laser differential confocal microscopy imaging［J］. Optical Technique, 2015, 41(5): 385-389.
16 舒攀, 盛忠, 邱丽荣, 等. 差动共焦显微成像高稳定三维扫描技术与系统［J］. 光学技术, 2017, 43(3): 212-216. ShuP, ShengZ, QiuL R, et al. Highly stable three-dimensional scanning technology and system in differential confocal microscopy imaging ［J］. Optical Technique, 2017, 43(3): 212-216.
17 程硕. 超分辨激光差动共焦微孔测量技术研究［D］. 北京: 北京理工大学, 2015.
18 王富生, 谭久彬. 差动共焦式纳米级光聚焦探测系统的研究［J］. 光学技术, 2001, 27(3): 232-234. WangF S, TanJ B. Optical focus detection system with nanometer resolution using differential confocal microscope［J］. Optical Technique, 2001, 27(3): 232-234.
19 尹哲. 差动共焦显微探测实验系统相关技术的研究［D］. 哈尔滨:哈尔滨工业大学, 2007.
20 张雪, 葛文琦, 余锦, 等. 基于现场可编程门阵列的脉冲单纵模激光器能量稳定控制技术［J］. 中国激光, 2017 ,(8): 82-89. ZhangX, GeW Q, YuJ. Energy Stability Control Technique for Pulsed Single-Longitude-Mode Laser Based on Field Program mable Gate Array［J］. Chinese Journal of Lasers, 2017, (8): 82-89.
21 张明倩. 透/反射式针尖增强拉曼光谱术关键技术与实验研究［D］. 北京: 清华大学, 2013.
22 周奇, 高思田, 刘炳锋, 等. 大范围二维纳米位移台的控制及非线性校准的实验研究［J］. 计量学报, 2018,39(6):771-776. ZhouQ, GaoS T, LiuB F, et al. Experimental Research on the Control and Non-linear Calibration of Large-scale Two-dimensional Nanometer Displacement Stage［J］. Acta Metrologica Sinica, 2018,39(6):771-776.
23 施慧, 高思田, 黄鹭, 等. 音叉式AFM探针针尖与表面接近特性的研究［J］. 计量学报, 2020, 待发表. ShiH, GaoS T, HuangL, et al. Research of Approaching Characteristics between the Tip and the Surface of Atomic Force Microscope based on Tuning Fork Probe［J］. Acta Metrologica Sinica, 2020. To be published.
24 施玉书, 连笑怡, 王艺瑄, 等. AFM扫描过程的模拟及针尖形状反求［J］. 计量学报, 2019, 40(2):177-182. ShiY S, LianX Y, WangY X, et al. Simiulation of AFM Scanning Process and Tip Shape Estimation［J］. Acta Metrologica Sinica, 2019, 40(2):177-182.
25 庞华峰, 姚辉璐, 杨庆怡, 等. 激光光镊拉曼光谱仪信噪比测量研究［J］. 物理与工程, 2008, 18(5): 24-26. PangH F, YaoH L, YangQ Y, et al. Signal-To-Noise Ratio Measurment Of The Laser Tweezers Raman Spectroscopy［J］. Physics And Engneering, 2008, 18(5): 24-26.
26 任玲玲, 赵迎春, 姚雅宣, 等. 几种代表性纯物质拉曼光谱有效测量程序的确定［J］. 现代测量与实验室管理, 2014, (6): 3-6. RenL L, ZhaoY C, YaoY X, et al. Determination of effective measurement procedures for Raman spectra of representative pure substances［J］. Advanced Measurement and Laboratory Management, 2014, (6): 3-6.