目标区域计算机自动识别是骨组织形态计量学的发展趋势。研究图像多特征信息,识别苏木素尹红染色病理切片中的骨小梁。分析骨组织病理切片HSL颜色空间通道的直方图特征,进行基于颜色通道的阈值分割,采用Lazy Snapping算法获得骨小梁深层次特征信息,改进扫描种子填充算法,使其融合目标区域多特征信息。实验结果表明改进的扫描种子填充算法将复杂图像骨小梁提取时间从常规软件的10多分钟提速到257ms。改进的扫描线算法,能快速获得稳健的目标区域。
Abstract
Target recognition is a development trend of bone histomorphometry. Study on the characteristics of image information, it is necessary to discriminate trabecular bone of bone tissue sliced by yin hung-hematoxylin. Analysis bone tissue image histogram channel characteristics of HSL color space, based on the image segmentation threshold of color channels, by Lazy Snapping method, it will obtain the image features of trabecular bone in-depth information, and it will improve scanning seed filling algorithm to extract multi feature information by fusion target area. The results show that extract trabecular bone by analysis image multi-feature let repetitive operation improve, improved scanning seed filling algorithm let complex image such as trabecular extraction speed from ten minutes to 257ms. Improved scan line algorithm can get the robust target area more quickly.
关键词
计量学 /
骨组织形态测量 /
病理切片 /
图像多特征发现 /
骨小梁识别
Key words
metrology /
bone histomorphometry /
pathological section /
image multi-feature found /
trabecular recognition
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参考文献
[［1］付鑫,马剑雄,董宝康,等.骨微结构检测方法研究进展［J］.中国骨与关节外科,2009,2(6):509-514.
［2］李建赤,黄必留,徐自强.骨组织形态计量学在临床骨病应用中的研究与进展［J］.中国组织工程研究与临床康复,2011,15(35):6607-6610.
［3］刘梅洁,李鸿泓,王少君,等.骨质疏松症脾肾两虚型病症结合动物模型的建立［J］.中国中医基础医学杂志,2012,(8):836-838
［4］张灿,戴如春.纳米压痕技术在骨组织微纳观结构研究中的应用［J］.中国医学物理学杂志,2011,28(6):3051-3054.
［5］李亚楠,刘红,刘梅洁,等.无比山药丸对去卵巢大鼠腰椎骨密度和股骨生物力学性能的影响［J］.中国中医基础医学杂志,2012,(10):1085-1086.
［6］Rodriguez-Lelis J M, Abundez-Pliego A, Vargas-Trevino M, et al. A Qualitative Stress Analysis of a Cross Section of the Trabecular Bone Tissue of a Distal Femur by Photoelasticity［J］.Bioinformatics and Biomedical Engineering, 2007,3: 422-525.
［7］曹立,雍宜民.老年股骨颈骨折患者骨密度、骨形态计量学的研究［J］.中华创伤杂志,2000,16(8):488-491.
［8］邱明才,杨宝恩,王觉英,等.正常国人髂骨形态计量参数的研究［J］.中华医学杂志, 1990,70(12):685-690.
［9］郭昭庆, 党耕町, 王志国,等.正常人髂嵴活检标本的骨组织形态计量学分析［J］.中华骨科杂志,1995, 15(4):228-230.
［10］邱明才,戴晨琳,李玉坤,等.代谢性骨病学［M］.北京:人民卫生出版社,2012,436-438.
［11］席越,孟淑琴,宫丽华,等.骨组织病理解剖学技术［M］.2版.北京:人民卫生出版社,2009,252-256
［12］Quest D, Gayer C, Hering P. Depth measurements of drilled holes in bone by laser triangulation for the field of oral implantology［J］.Journal of Applied Physics, 2012, 111(1):013106.
［13］Gundersen H J G, Jensen T B, Osterby R. Distribution of membrane thickness determined by lineal analysis［J］.Journal of Microscopy, 1978, 113(1):27-43.
［14］Ekaterina N,Chen P Y, Elham H,et al. Recent advances on the measurement and calculation of the elastic moduli of cortical and trabecular bone:A review［J］.Theoretical and Applied Mechanics.2011,38(3): 209-297.
［15］Khamees J, Darwiche M A, Kochaji N. Alveolar ridge augmentation using chin bone graft, bovine bone mineral, and titanium mesh: Clinical, histological, and histomorphomtric study［J］.Journal of Indian Society of periodontology,2012, 16(2):235-240.
［16］Van't Hof R J, Landao-Basonga E.Semi- automated histomorphometry of bone resorption parameters［J］.Bone, 2011,48(2): S135-S136.
［17］Osteo Measure TM software［OL］.http://www.osteometrics.com/product_info.htm.2015-01-06.
［18］Bioquant OsteoⅡ software［OL］. http://osteo.bioquant.com/home.2015-01-06.
［19］Droke A E, Hager A K, Lerner R M, et al. Soy isoflavones avert chronic inflammation induced bone loss and vascular disease［J］. Journal of Inflammation, 2007, 4 (1):17-29.
［20］叶含笑,金红婷,袁昕,等.基于视觉模型的苏木精-伊红染色法染色病理切片骨小梁提取［J］.计量学报,2014, 35(3):291-295.
［21］Darabi A, Chandelier F, Baroud G. Morphometric analysis of trabecular bone thickness using different algorithms［J］. Can J Elect Comput Eng,2007, 32(3):157-163.
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