100μL微量移液器国际关键比对的数学模型研究

佟林,王金涛,刘子勇,魏凯,张珑,郭立功,赵晓惠

计量学报 ›› 2015, Vol. 36 ›› Issue (2) : 154-157.

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PDF(378 KB)
计量学报 ›› 2015, Vol. 36 ›› Issue (2) : 154-157. DOI: 10.3969/j.issn.1000-1158.2015.02.10

100μL微量移液器国际关键比对的数学模型研究

  • 佟林1,王金涛1,刘子勇1,魏凯2,张珑1,郭立功1,赵晓惠1
作者信息 +

The Research of Mathematical Models in BIPM/CIPM Key Comparison CCM.FF-K4.2.2011 Volume Comparison at 100 μL

  • TONG Lin1,WANG Jin-tao1,LIU Zi-yong1,WEI Kai2,ZHANG Long1,GUO Li-gong1,ZHAO Xiao-hui1
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文章历史 +

摘要

为验证各国对微量移液器的校准能力,组织了国际关键比对。通过比对发现大气压力对移液器的校准结果有着较大影响,为此对数学模型进行了修正,经过修正后计算所得各参比实验室的校准结果有了较好的一致性。同时通过大量实验得出,微量移液器由于其自身结构存在不稳定性所带入的不确定度分量是不确定度分析中的一个主要来源。比对中研究所得的数学模型其算法准确、结果可靠,很好地解决了移液器在日常检定及校准中的数据处理问题。

Abstract

According the key comparison found that the atmospheric pressure has a great impact on the pipette calibration results, and after pressure correction there is a better consistency in report. On the other hand the micropipette reliability is the major source of uncertainty on the total calibration uncertainty through a lot of laboratory tests. Finally the mathematical models are proven to be accurate and reliable through a numerical example based on the key comparison, and they could be used in calibration and data processing for micropipettes.

关键词

计量学 / 微量移液器 / 关键比对 / 静力称重法 / 不确定度 / 压力修正

Key words

Metrology / Micropipette / Key comparison / Gravimetric method / Uncertainty / Pressure correction

引用本文

导出引用
佟林,王金涛,刘子勇,魏凯,张珑,郭立功,赵晓惠. 100μL微量移液器国际关键比对的数学模型研究[J]. 计量学报. 2015, 36(2): 154-157 https://doi.org/10.3969/j.issn.1000-1158.2015.02.10
TONG Lin,WANG Jin-tao,LIU Zi-yong,WEI Kai,ZHANG Long,GUO Li-gong,ZHAO Xiao-hui. The Research of Mathematical Models in BIPM/CIPM Key Comparison CCM.FF-K4.2.2011 Volume Comparison at 100 μL[J]. Acta Metrologica Sinica. 2015, 36(2): 154-157 https://doi.org/10.3969/j.issn.1000-1158.2015.02.10

参考文献

1]刘子勇,张珑,郭立功,等. 容量计量[M]. 北京:中国计量出版社,2009,108-122.
2]Tanaka M, et al. Recommended table for the density of water between 0℃ and 40℃ based on recent experimental reports[J].Metrologia,2001,38:301-309.
3]The International Organization for Standardization ISO4787:2010,Laboratory glassware-Volumetric glassware-Methods for use and testing of capacity[S]. 2010.
4]The International Organization for Standardization ISO8655-1/2/6:2002, Piston-operated volumetric apparatus[S].2002.
5]Lorefice S. Traceability and uncertainty analysis in volume measurements[J].Measurement,2009,42:1510-1515.
6]国家质量技术监督局. JJF 1059—1999 测量不确定度评定与表示[S].1999.
7]佟林,王金涛,刘子勇,等. 移液器使用及校准过程中影响因素分析[J]. 中国计量,2012,(5):79-81.
8]Physikalisch-Technische Bundesanstalt DKD-R 8-1,Calibration of piston pipettes[S].2011.

基金

国家自然科学基金项目(51105347);
国家质检总局量值传递资助项目(22-ALC1004)

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