西洋参中人参皂苷Rg1、Re、Rb1基体标准物质的研制

任中楠,刘军,黄挺,杨屹

计量学报 ›› 2013, Vol. 34 ›› Issue (2) : 187-191.

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PDF(601 KB)
计量学报 ›› 2013, Vol. 34 ›› Issue (2) : 187-191. DOI: 10.3969/j.issn.1000-1158.2013.02.18

西洋参中人参皂苷Rg1、Re、Rb1基体标准物质的研制

  • 任中楠1,刘军2,黄挺2,杨屹1
作者信息 +

Development of a New Certified Reference Material of Ginsenoside Rg1, Re and Rb1 in Panax Quinquefolium

  • REN Zhong-nan1,LIU Jun2,HUANG Ting2,YANG Yi1
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文章历史 +

摘要

建立高效液相色谱法和高效毛细管电泳法,对研制的西洋参基体标准物质中人参皂苷Rg1、Re、Rb1含量进行准确定值,同时考察了样品的均匀性、稳定性和样品的最小取样量,最后对标准物质的不确定度进行了评定。评定结果为人参皂苷Rg1、Re、Rb1含量分别为(1.72±0.14)mg/g、(15.3±1.5)mg/g、(25.8±2.8)mg/g,相对扩展不确定度分别为8.4%、10%、11%,可为西洋参的质量控制提供依据。

Abstract

A new certified reference material (CRM) of ginsenoside Rg1, Re, and Rb1 in Panax quinquefolium is developed and measured by two different methods, high performance liquid chromatography method and high performance capillary electrophoresis method.Simultaneously, the homogeneity and the stability are studied, and the evaluation of uncertainty is calculated.  The mass fractions of ginsenoside Rg1, Re, and Rb1 are (1.72±0.14)mg/g, (15.3±1.5)mg/g, (25.8±2.8)mg/g, with the expanded uncertainty of 8.3%, 10%, 11%.The new certified reference material of ginsenoside Rg1, Re, and Rb1  in Panax quinquefolium is capable to validate analytical measurements  in laboratories.

关键词

计量学 / 标准物质 / 西洋参 / 人参皂苷Rg1 / 人参皂苷Re / 人参皂苷Rb1

Key words

Metrology / Standard certified reference material / Panax quinquefolium / Ginsenoside Rg1 / Ginsenoside Re / Ginsenoside Rb1

引用本文

导出引用
任中楠,刘军,黄挺,杨屹. 西洋参中人参皂苷Rg1、Re、Rb1基体标准物质的研制[J]. 计量学报. 2013, 34(2): 187-191 https://doi.org/10.3969/j.issn.1000-1158.2013.02.18
REN Zhong-nan,LIU Jun,HUANG Ting,YANG Yi. Development of a New Certified Reference Material of Ginsenoside Rg1, Re and Rb1 in Panax Quinquefolium[J]. Acta Metrologica Sinica. 2013, 34(2): 187-191 https://doi.org/10.3969/j.issn.1000-1158.2013.02.18

参考文献

[1]周婧,都晓伟,马宏跃,等.西洋参药材高效液相色谱法指纹图谱的研究[J].时珍国医国药,2006,17(12): 2381-2382.
[2]Bai Y P, Zhao L S, Qu C L, et al.Microwave degradation of floatation-enriched ginsenoside extract from Panax quinquefolium L.leaf[J].J Agric Food Chem,2009,57(21): 10252-10260.
[3]Wang Y T, You J Y, Yu Y ,et al.Analysis of ginsenosides in Panax ginseng in high pressure microwave-assisted extraction[J].Food Chemistry, 2008,110(1):161-167.
[4]杨伟峰,赵维良.胶束电动毛细管色谱法测定西洋参中人参皂昔Rb1, Re的含量[J].中国中药,2003,28(12):1135-1137.
[5]郑晓晖,赵新锋,胡震,等.液相色谱-离子阱质谱联用法建立留坝西洋参药材特征图谱研究[J].中草药,2005,36(1):104-106.
[6]全浩,韩永志.标准物质及其应用技术[M].北京:中国标准出版社,2003.
[7]魏昊.化学分析中不确定度的评估指南[M].北京:中国计量出版社, 2002.

基金

科技部科技基础工作专项(2008FY130200)

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