转基因大豆 Mon89788 基体标准物质协同实验研究

张玲; 盛灵慧; 王晶; 曹应龙; 柳方方; 隋志伟; 卢长明

doi:10.3969/j.issn.1000-1158.2011.z1.27

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计量学报 ›› 2011, Vol. 32 ›› Issue (z1) : 106-109. DOI: 10.3969/j.issn.1000-1158.2011.z1.27

转基因大豆 Mon89788 基体标准物质协同实验研究

张玲¹,盛灵慧¹,王晶¹,曹应龙²,柳方方³,隋志伟¹,卢长明²

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Cooperative Verification of the Value of GM Soybean Mon89788 Matrix Reference Materials

ZHAG Ling¹,SHENG Ling-hui¹,WANG Jing¹,CAO Ying-long²,LIU Fang-fang³,SUI Zhi-wei¹,LU Chang-ming²

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文章历史 +

摘要

对研制的3个含量水平的转基因大豆Mon89788 基体标准物质进行多家实验室的验证研究。研究过程包括转基因大豆中基因组DNA的提取和转基因组分的定量PCR方法确认,标准物质的多家实验室协同实验,以及测量的不确定度评估。多家测定分析结果表明,转基因大豆Mon89788 3种不同外源基因/内源基因含量水平的标准物质参考值和扩展不确定度分别为 0.35%±0.17%,0.61%±0.14%,3.17%±1.70% (k=2)。基体为大豆粉的该标准物质可用于转基因大豆含量的定量检测。

Abstract

Three levels of genetically modified soybeans Mon89788 matrix reference material are developed. Genetically modified soybean genomic DNA extraction and quantitative PCR of transgenic components methods are researched. A multi-laboratory verification and uncertainty evaluation are carried out too.The analysis of multi-laboratory value verification shows that in three different levels of genetically modified soybean Mon89788 reference materials, the transgenic gene / endogenous genes reference value and uncertainty is 0.35%±0.17%,0.61%±0.14%,3.17%±1.70% (k=2), respectively.This set of reference materials that the matrix is soybean grand can be used for the quantitative detection of genetically modified soybeans content.

导出引用

张玲,盛灵慧,王晶,曹应龙,柳方方,隋志伟,卢长明. 转基因大豆 Mon89788 基体标准物质协同实验研究[J]. 计量学报. 2011, 32(z1): 106-109 https://doi.org/10.3969/j.issn.1000-1158.2011.z1.27

ZHAG Ling,SHENG Ling-hui,WANG Jing,CAO Ying-long,LIU Fang-fang,SUI Zhi-wei,LU Chang-ming. Cooperative Verification of the Value of GM Soybean Mon89788 Matrix Reference Materials[J]. Acta Metrologica Sinica. 2011, 32(z1): 106-109 https://doi.org/10.3969/j.issn.1000-1158.2011.z1.27

中图分类号： TB99

参考文献

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