Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China
Abstract：Anoikis effect is a special program death for attachment cells in the body, which plays an important role in body development, tissue homeostasis, disease occurrence, and tumor metastasis. Attachment epithelial cell line Hela was hired as the experiment model to investigate the anoikis effect in detail. Living Hela cells were counted after trypsinization and floating for 5min, 30min, 1h, 2h, 4h, and 8h respectively. The activity of these cells was detected after seeding back for 24h. The results showed that floating time would severely decrease cell counting and activity of HeLa, and the floating time of attachment cell needs to be controlled during cell experiments.
Desdouets C, Sobczak-thépot J, Murphy M, et al. Cyclin A: function and expression during cell proliferation ［J］. Prog Cell Cycle Res, 1995(1): 115-123.
Mirabelli P, Coppola L, Salvatore M. Cancer Cell Lines Are Useful Model Systems for Medical Research ［J］. Cancers (Basel), 2019 , 11(1098): 1-18.
［2］
Wong C H, Siah K W, Lo A W. Estimation of clinical trial success rates and related parameters ［J］. Biostatistics, 2019, 20(2): 273-286.
［4］
Faruqui N, Kummrow A, Fu B, et al. Cellular Metrology: Scoping for a Value Proposition in Extra-and Intracellular Measurements ［J］. Front Bioeng Biotechnol, 2020, 7: 1-8.
［5］
金力. 计量测试赋能生命科学高质量发展［J］. 质量与标准化, 2021(6): 1-4.
Xue Z C, Zeng J M, Li Y S, et al. Cell Growth and Drug Effect Differences Induced by Inoculum Number Variations and Comparison of Cell Counting Methods［J］. Acta Metrologica Sinica, 2023, 44(3): 341-349.
［11］
Cai Q, Yan L, Xu Y. Anoikis Resistance Is a Critical Feature of Highly Aggressive Ovarian Cancer Cells ［J］. Oncogene, 2014, 34(25): 3315-3324.
［12］
Peppicelli S, Ruzzolini J, Bianchini F, et al. Anoikis Resistance as a Further Trait of Acidic-adapted Melanoma Cells ［J］. Journal of Oncology, 2019(25): 1-13.
［15］
Wu Y, Wu M, He G, et al. Glyceraldehyde-3-phosphate Dehydrogenase: a Universal Internal Control for Western Blots in Prokaryotic and Eukaryotic Cells［J］. Anal Biochem, 2012, 423(1): 15-22.
［17］
Chang J C. Cancer Stem Cells: Role in Tumor Growth, Recurrence, Metastasis, and Treatment Resistance ［J］. Medicine (Baltimore), 2016, 95(Suppl 1): 20-25.
［3］
Amelian A, Wasilewska K, Megias D, et al. Application of standard cell cultures and 3D in vitro tissue models as an effective tool in drug design and development ［J］. Pharmacological Reports, 2017, 69(5): 861-870.
［10］
Paoli P, Giannoni E, Chiarugi P. Anoikis Molecular Pathways and Its Role in Cancer Progression ［J］. Biochim Biophys Acta, 2013, 1833(12): 3481-3498.
Liu J M, Jia X Q, Di B. Application of three-dimensional cell model in tumor research ［J］. J China Pharm Univ, 2020, 51(2): 152-160.
［7］
Scherer W F, Syverton J T, Gey G O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix ［J］. J Exp Med, 1953, 97(5): 695-710.
［16］
Akekawatchai C, Roytrakul S, Phaonakrop N, et al. Proteomic Analysis of the Anoikis-resistant Human Breast Cancer Cell Lines ［J］. Methods Mol Biol, 2020, 2138: 185-193
［18］
Huyen N T B, Prachayasittikul V, Chan-on W. Anoikis-resistant Cholangiocarcinoma Cells Display Aggressive Characteristics and Increase Stat3 Activation. ［J］. J Hepatobiliary Pancreat Sci, 2016, 23(7): 397-405.
2023, Vol. 44 
