枯草芽孢杆菌黑色变种芽孢测量方法研究

doi:10.3969/j.issn.1000-1158.2020.09.21

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摘要枯草芽孢杆菌黑色变种芽孢是生物防护和灭菌效果评价的重要指示微生物,其量值准确可以保证评价的有效性和可比性。对枯草芽孢杆菌涂布平板法进行了方法验证和协同实验验证以及不确定度分析。结果显示,当平板上的菌落数在30～300CFU之间时,该方法重复性和再现性均较好,室内重复性相对标准偏差为10.0%,室间相对标准偏差为14.1%。经统计分析,胰蛋白胨大豆琼脂培养基平板涂布法相对扩展不确定度为12.0%(k=2),适用于枯草芽孢杆菌黑色变种芽孢定量测量,对于人员防护装备防护性能评价、消毒剂检测及灭菌质量控制具有重要意义。

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	隋志伟
	王斌
	刘思渊
	王晶
	傅博强
	卓婷烨
	王义

关键词 ：计量学;枯草芽孢杆菌黑色变种;芽孢;涂布平板法;胰蛋白胨大豆琼脂, 协同实验验证

Abstract：Bacillus subtilis var.niger is an important indicator microbiology of biological protection and sterilization effect evaluation, and its accurate value can ensure the effectiveness and comparability of the evaluation. Spread plate method for bacillus subtilis was method validated, collaborative experimental verified, and uncertainty analyzed. The results showed that spread plate method has a good reproducibility and repeatability, within-laboratory relative standard deviation of repeatability is 10.0%, and inter-laboratory relative standard deviations is 14.1%, when the number of colonies on the plate is between 30CFU and 300CFU. The statistic test showed that the relative expanded uncertainty of spread plate method is 12.0% (k=2). This method can be used for quantitative measurement of bacillus subtilis var. niger spore, which has important significance for the evaluation of protective performance of personnel protective equipment, detection of disinfectants and quality control of sterilization.

Key words： metrology bacillus subtilis var.niger spores spread plate method tryptone soy agar collaborative experimental verification

收稿日期: 2018-09-14 发布日期: 2020-08-28

PACS:

TB99

基金资助:国家重点研发计划项目(2017YFF0204602, 2018YFC1200501);公益性科研院所基本科研业务费专项(AKY1818-18)

作者简介: 隋志伟(1980-),山西太原人,中国计量科学研究院副研究员,博士,主要从事微生物计量标准研究。Email:suizhw@nim.ac.cn

引用本文:

隋志伟,王斌,刘思渊,王晶,傅博强,卓婷烨,王义. 枯草芽孢杆菌黑色变种芽孢测量方法研究[J]. 计量学报, 2020, 41(9): 1171-1176.

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